Monday, 4 August 2014

Stopping gliadin peptides in their tracks?

A micropost if you will, to bring to your attention the paper by Marco Sarno and colleagues [1] and their suggestion of "a novel effect of probiotics in the prevention of undigested gliadin peptides toxic effects".
Knitting on't Moors @ Wikipedia 

The probiotic in question is something called Lactobacillus paracasei CBA L74, which if I'm not mistaken is connected with a certain '57 varieties' company. The authors (which included one Alessio Fasano) indicated that said probiotic appeared to inhibit two gliadin peptides, P31-43 and P57-68, entrance in Caco2 cells, a model of the intestinal barrier [2]. Given that the entrance of such gliadin peptides into the gut mucosa (well the lamina propria) is part of the autoimmune condition known as coeliac (celiac) disease (see here), one might reasonably assume that use of Lactobacillus paracasei CBA L74 (LP CBA L74) might hold some potential as a possible treatment strategy outside of the standard gluten-free diet. At the very least, assuming some further investigations are indicated, it might be added to the list of other compounds being tested with coeliac disease in mind (see here).

I note that this is not the first time that LP CBA L74 has come under the peer-reviewed research spotlight as per the paper by Elena Zagato and colleagues [3] (open-access here). On that occasion the authors reported that: "fermented products of Lactobacillus paracasei CBA L74 act via the inhibition of proinflammatory cytokine release" and "could protect against colitis and against an enteric pathogen infection". With those statements in mind, I wonder whether coeliac disease might just be the tip of the iceberg when it comes to the applications for LP CBA L74 as per the work of a distant relation?

Speaking of tracks, here's Smokey and the Tracks of My Tears.

----------

[1] Sarno M. et al. Lactobacillus paracasei CBA L74 interferes with gliadin peptides entrance in Caco-2 cells. Int J Food Sci Nutr. 2014 Jul 17:1-7.

[2] Sambuy Y. et al. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol. 2005 Jan;21(1):1-26.

----------

ResearchBlogging.org Sarno M, Lania G, Cuomo M, Nigro F, Passannanti F, Budelli A, Fasano F, Troncone R, Auricchio S, Barone MV, Nigro R, & Nanayakkara M (2014). Lactobacillus paracasei CBA L74 interferes with gliadin peptides entrance in Caco-2 cells. International journal of food sciences and nutrition, 1-7 PMID: 25030417